Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- sc-9976 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-9976, RRID:AB_627502
- Product name
- Anti-EIF4E
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Dual mechanism for the translation of subgenomic mRNA from Sindbis virus in infected and uninfected cells.
RAS–RAF–MEK-dependent oxidative cell death involving voltage-dependent anion channels
Sanz MA, Castelló A, Ventoso I, Berlanga JJ, Carrasco L
PloS one 2009;4(3):e4772
PloS one 2009;4(3):e4772
RAS–RAF–MEK-dependent oxidative cell death involving voltage-dependent anion channels
Nicholas Yagoda, Moritz von Rechenberg, Elma Zaganjor, Andras J. Bauer, Wan Seok Yang, Daniel J. Fridman, Adam J. Wolpaw, Inese Smukste, John M. Peltier, J. Jay Boniface, Richard Smith, Stephen L. Lessnick, Sudhir Sahasrabudhe, Brent R. Stockwell
Nature 2007 Jun;447(7146):865-869
Nature 2007 Jun;447(7146):865-869
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 25
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.