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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10633 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10633, RRID:AB_11189063
- Product name
- EIF4E monoclonal antibody, clone 5D11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant EIF4E.
- Isotype
- IgG
- Antibody clone number
- 5D11
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) against HeLa (1) , HEK293 (2) and K-562 (3) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) .
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GC-7901 cells using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer (A) , submaxillary tumor (B) , stomach cancer (C) and prostate cancer (D) using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using EIF4E monoclonal antibody, clone 5D11 (Cat # MAB10633) (green) and negative control (purple) .
- Validation comment
- Flow Cytometry
