693003

antibody from BioLegend

Targeting: EIF4E EIF4E1, EIF4EL1, EIF4F

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

693003

Provider

BioLegend

Proper citation

BioLegend Cat#693003, RRID:AB_2650791

Product name

Alexa Fluor® 594 anti-eIF4E

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

P85D11B4

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.5 µg/mL of Alexa Fluor® 594 anti-eIF4E antibody (clone P85D11B4) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye