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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-109681 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF4E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Sequence of this protein is as follows: MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.48 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF4E in HL-60, SW620, MCF-7, 293T, mouse brain, rat testis. Samples were incubated in polyclonal eIF4E antibody (Product # PA5-109681) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF4E in HL-60, SW620, MCF-7, 293T, mouse brain, rat testis. Samples were incubated in polyclonal eIF4E antibody (Product # PA5-109681) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of eIF4E in U-2OS cells. Samples were incubated in polyclonal eIF4E antibody (Product # PA5-109681) using a dilution of 1:100, followed by DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF4E in paraffin-embedded human lung cancer tissue. Samples were incubated in polyclonal eIF4E antibody (Product # PA5-109681) using a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF4E in paraffin-embedded human stomach tissue. Samples were incubated in polyclonal eIF4E antibody (Product # PA5-109681) using a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of eIF4E in 293T cells. Samples were incubated in polyclonal eIF4E antibody (Product # PA5-109681) using a dilution of 1:1000.
