Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP2-34122 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Helios Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Helios antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Helios Antibody [NBP2-34122] - Analysis in human cell line RT-4 and human cell line SK-MEL-30.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Helios Antibody [NBP2-34122] - Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Helios Antibody [NBP2-34122] - Staining of human lymphoid tissues shows moderate to strong nuclear positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Helios Antibody [NBP2-34122] - Staining of human esophagus shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Helios Antibody [NBP2-34122] - Analysis in human esophagus and cerebral cortex tissues. Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Helios Antibody [NBP2-34122] - Staining of human squamous epithelia shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Helios Antibody [NBP2-34122] - Staining of human cerebral cortex shows no positivity in neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Helios Antibody [NBP2-34122] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.