17-3079-42

antibody from Invitrogen Antibodies

Targeting: FCRL4 CD307d, FCRH4, IGFP2, IRTA1

Provider product page for 17-3079-42

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [3]
Comments [0]
Validations
Flow cytometry [2]
Other assay [4]

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Product number: 17-3079-42 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD307d (FcRL4) Monoclonal Antibody (413D12), APC, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: The 413D12 monoclonal antibody reacts with human CD307d, which is also known as FcRL4, Fc receptor-like 4, FcRH4, IRTA4, or IGFP2. CD307d is an inhibitory receptor that is highly expressed on a subset of memory B cells resident in epithelial tissues and marginal zones of mucosal lymphoid tissues such as tonsils. CD307d has been shown to recruit the phosphatase SHP-1, thereby acting as an inhibitory receptor in B cells. CD307d is homologous to FcgammaRI and specifically binds IgA. To date, CD307d is the only known inhibitory receptor for IgA. Expression of CD307d is increased on memory B cells from patients with chronic infections such as HIV, suggesting that enhanced expression of inhibitory receptors, such as CD307d, may contribute to the inefficiency of antibody responses against HIV. Applications Reported: This 413D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 413D12 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells and transfected 293T cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 413D12
Vial size: 100 Tests
Concentration: 5 µL/Test
Storage: 4° C, store in dark, DO NOT FREEZE!

FCRL4 protein structure - 17-3079-42 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. RUNX1 responsiveness of the FCRL4 gene promoter. (A) Analysis of the putative promoter sequence of the FCRL4 gene. Locations are indicated for potential RUNX (open circles) and SOX5 (closed triangle) binding sites. (B) Luciferase reporter gene studies on transiently transfected 293T cells. Cells were transfected with the putative promoter of the FCRL4 gene controlling expression of the luciferase reporter gene and expression vectors containing the indicated transcription factors. RUNX2 Delta5 and RUNX2 Delta5,7 are splice isoforms that lack exon 5 and exons 5 and 7, respectively (see also Fig. S1, available at http://www.jem.org/cgi/content/full/jem.20072682/DC1 ). Values represent mean +- SD ( n = 5). (C) ChIP assay of RUNX1 binding in FACS-sorted FCRL4 + and FCRL4 - B Mem cells. Positions of the amplified PCR fragments are indicated by closed rectangles in A. Open bars indicate signals obtained from FCRL4 + cells, and shaded bars indicate signals obtained from FCRL4 - cells. All values were normalized to the PCR signal obtained from control immunoprecipitates (anti-SHP-1), as well as to unrelated, nonspecifically copurified DNA (CCR4). Values represent mean +- SEM ( n = 5). Statistical significance of P < 0.05 is indicated by an asterisk.