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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00055686-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055686-M01, RRID:AB_538380
- Product name
- Mouse Monoclonal DSU Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DSU - likely ortholog of mouse dilute suppressor
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Loss of melanoregulin (MREG) enhances cathepsin-D secretion by the retinal pigment epithelium.
Melanoregulin, product of the dsu locus, links the BLOC-pathway and OA1 in organelle biogenesis.
Frost LS, Lopes VS, Stefano FP, Bragin A, Williams DS, Mitchell CH, Boesze-Battaglia K
Visual neuroscience 2013 May;30(3):55-64
Visual neuroscience 2013 May;30(3):55-64
Melanoregulin, product of the dsu locus, links the BLOC-pathway and OA1 in organelle biogenesis.
Rachel RA, Nagashima K, O'Sullivan TN, Frost LS, Stefano FP, Marigo V, Boesze-Battaglia K
PloS one 2012;7(9):e42446
PloS one 2012;7(9):e42446
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DSU Antibody (8F9-1B2) [H00055686-M01] - DSU monoclonal antibody (M01), clone 8F9-1B2 Western Blot analysis of DSU expression in MCF-7 ( Cat # L046V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DSU Antibody (8F9-1B2) [H00055686-M01] - Analysis of DSU expression in transfected 293T cell line by DSU monoclonal antibody (M01), clone 8F9-1B2.Lane 1: DSU transfected lysate(25 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DSU Antibody (8F9-1B2) [H00055686-M01] - Detection limit for recombinant GST tagged DSU is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DSU Antibody (8F9-1B2) [H00055686-M01] - Analysis of MREG transfected lysate using anti-MREG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MREG MaxPab rabbit polyclonal antibody.
