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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX109468 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109468, RRID:AB_11179132
- Product name
- SmarcAL1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A549 5% SDS PAGE GTX109468 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SmarcAL1 antibody (GTX109468) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using SmarcAL1(GTX109468) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SmarcAL1 antibody immunoprecipitates SMARCAL1 protein in IP experiments.IP samples: A549 whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of SMARCAL1 protein by 4 £gg SmarcAL1 antibody (GTX109468)5 % SDS-PAGEThe immunoprecipitated SMARCAL1 protein was detected by SmarcAL1 antibody (GTX109468) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
