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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-28980 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCAL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, mouse testis, rat testis. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.07 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SmarcAL1 using 30 µg of A549 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a SmarcAL1 polyclonal antibody (Product # PA5-28980) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SMARCAL1 was performed by separating 30 µg of K562 whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SMARCAL1 Polyclonal Antibody (Product # PA5-28980) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SMARCAL1 Polyclonal Antibody (Product # PA5-28980). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCAL1 Polyclonal Antibody (Product # PA5-28980) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SMARCAL1 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SMARCAL1 Polyclonal Antibody (Product # PA5-28980) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SMARCAL1 was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SMARCAL1 Polyclonal Antibody (Product # PA5-28980) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SmarcAL1 in paraformaldehyde-fixed A549 cells using a SmarcAL1 polyclonal antibody (Product # PA5-28980) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMARCAL1 Polyclonal Antibody immunoprecipitates SMARCAL1 protein in IP experiments. IP samples: A549 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of SMARCAL1 protein by 4 µg SMARCAL1 Polyclonal Antibody (Product # PA5-28980). 5 % SDS-PAGE. The immunoprecipitated SMARCAL1 protein was detected by SMARCAL1 Polyclonal Antibody (Product # PA5-28980) diluted at 1:1,000.
