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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09243PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09243PU-N, RRID:AB_2035970
- Product name
- anti SMARCAL1 / HARP (80-96)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding aa 80-96 of human SmarcAL1 protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 2.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using anti-SmarcAL1 antibody shows detection of a band ~106 kDa band (arrowhead) corresponding to SmarcAL1 in human derived cultured cell lysates (HeLa nuclear extract lane 1, HeLa lane 2, A431 lane 3, Jurkat lane 4, and 293 lane 5). Approximately 5 μg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers.
