Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF385 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PDGF R beta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PDGF R beta is observed, and less than 1% cross-reactivity with recombinant human PDGF sR alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Blood brain barrier leakage is not a consistent feature of white matter lesions in CADASIL.
The amyloid-β degradation intermediate Aβ34 is pericyte-associated and reduced in brain capillaries of patients with Alzheimer's disease.
Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-β42 by LRP1-dependent apolipoprotein E isoform-specific mechanism.
Reductions in brain pericytes are associated with arteriovenous malformation vascular instability.
Transcriptome analysis of PDGFRα+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFRα+ cell hyperplasia.
A tissue-specific role for intraflagellar transport genes during craniofacial development.
Coculture of endothelial progenitor cells and mesenchymal stem cells enhanced their proliferation and angiogenesis through PDGF and Notch signaling.
Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
Growth factor receptor-Src-mediated suppression of GRK6 dysregulates CXCR4 signaling and promotes medulloblastoma migration.
PDGFRβ expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model.
Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis.
Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis.
Rajani RM, Ratelade J, Domenga-Denier V, Hase Y, Kalimo H, Kalaria RN, Joutel A
Acta neuropathologica communications 2019 Nov 21;7(1):187
Acta neuropathologica communications 2019 Nov 21;7(1):187
The amyloid-β degradation intermediate Aβ34 is pericyte-associated and reduced in brain capillaries of patients with Alzheimer's disease.
Kirabali T, Rigotti S, Siccoli A, Liebsch F, Shobo A, Hock C, Nitsch RM, Multhaup G, Kulic L
Acta neuropathologica communications 2019 Dec 3;7(1):194
Acta neuropathologica communications 2019 Dec 3;7(1):194
Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-β42 by LRP1-dependent apolipoprotein E isoform-specific mechanism.
Ma Q, Zhao Z, Sagare AP, Wu Y, Wang M, Owens NC, Verghese PB, Herz J, Holtzman DM, Zlokovic BV
Molecular neurodegeneration 2018 Oct 19;13(1):57
Molecular neurodegeneration 2018 Oct 19;13(1):57
Reductions in brain pericytes are associated with arteriovenous malformation vascular instability.
Winkler EA, Birk H, Burkhardt JK, Chen X, Yue JK, Guo D, Rutledge WC, Lasker GF, Partow C, Tihan T, Chang EF, Su H, Kim H, Walcott BP, Lawton MT
Journal of neurosurgery 2018 Dec 1;129(6):1464-1474
Journal of neurosurgery 2018 Dec 1;129(6):1464-1474
Transcriptome analysis of PDGFRα+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFRα+ cell hyperplasia.
Ha SE, Lee MY, Kurahashi M, Wei L, Jorgensen BG, Park C, Park PJ, Redelman D, Sasse KC, Becker LS, Sanders KM, Ro S
PloS one 2017;12(8):e0182265
PloS one 2017;12(8):e0182265
A tissue-specific role for intraflagellar transport genes during craniofacial development.
Schock EN, Struve JN, Chang CF, Williams TJ, Snedeker J, Attia AC, Stottmann RW, Brugmann SA
PloS one 2017;12(3):e0174206
PloS one 2017;12(3):e0174206
Coculture of endothelial progenitor cells and mesenchymal stem cells enhanced their proliferation and angiogenesis through PDGF and Notch signaling.
Liang T, Zhu L, Gao W, Gong M, Ren J, Yao H, Wang K, Shi D
FEBS open bio 2017 Nov;7(11):1722-1736
FEBS open bio 2017 Nov;7(11):1722-1736
Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
Rizvi S, Mertens JC, Bronk SF, Hirsova P, Dai H, Roberts LR, Kaufmann SH, Gores GJ
The Journal of biological chemistry 2014 Aug 15;289(33):22835-49
The Journal of biological chemistry 2014 Aug 15;289(33):22835-49
Growth factor receptor-Src-mediated suppression of GRK6 dysregulates CXCR4 signaling and promotes medulloblastoma migration.
Yuan L, Zhang H, Liu J, Rubin JB, Cho YJ, Shu HK, Schniederjan M, MacDonald TJ
Molecular cancer 2013 Mar 5;12:18
Molecular cancer 2013 Mar 5;12:18
PDGFRβ expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
Hewitt KJ, Shamis Y, Knight E, Smith A, Maione A, Alt-Holland A, Sheridan SD, Haggarty SJ, Garlick JA
Journal of cell science 2012 May 1;125(Pt 9):2276-87
Journal of cell science 2012 May 1;125(Pt 9):2276-87
Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM
The Journal of biological chemistry 2009 Jun 12;284(24):16693-16703
The Journal of biological chemistry 2009 Jun 12;284(24):16693-16703
Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model.
Miyake T, Aoki M, Morishita R
Cardiovascular research 2008 Sep 1;79(4):706-14
Cardiovascular research 2008 Sep 1;79(4):706-14
Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
Cancer research 2008 Apr 1;68(7):2085-93
Cancer research 2008 Apr 1;68(7):2085-93
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Ball SG, Shuttleworth CA, Kielty CM
The Journal of cell biology 2007 May 7;177(3):489-500
The Journal of cell biology 2007 May 7;177(3):489-500
C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
Blood 2007 May 15;109(10):4264-71
Blood 2007 May 15;109(10):4264-71
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Li R, Maminishkis A, Wang FE, Miller SS
Investigative ophthalmology & visual science 2007 Dec;48(12):5722-32
Investigative ophthalmology & visual science 2007 Dec;48(12):5722-32
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 May;31(5):516-22
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2006 May;31(5):516-22
Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
Blood 2006 Mar 1;107(5):1896-902
Blood 2006 Mar 1;107(5):1896-902
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
The American journal of pathology 2003 Sep;163(3):869-78
The American journal of pathology 2003 Sep;163(3):869-78
Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis.
Campbell M, Allen WE, Silversides JA, Trimble ER
Diabetes 2003 Feb;52(2):519-26
Diabetes 2003 Feb;52(2):519-26
Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis.
Campbell M, Allen WE, Silversides JA, Trimble ER
Diabetes 2003 Feb;52(2):519-26
Diabetes 2003 Feb;52(2):519-26
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PDGF R beta in Human Breast Cancer Tissue. PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PDGF R beta Inhibition of PDGF-BB-dependent Cell Proliferation and Neutralization by Human PDGF R beta Antibody. Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) inhibits Recombinant Human PDGF-BB (Catalog # 220-BB) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND50 is typically 10-40 µg/mL.
