- MA5-15143 - Invitrogen Antibodies PDGFRB antibody

Submitted references Improving hindlimb locomotor function by Non-invasive AAV-mediated manipulations of propriospinal neurons in mice with complete spinal cord injury.

Brommer B, He M, Zhang Z, Yang Z, Page JC, Su J, Zhang Y, Zhu J, Gouy E, Tang J, Williams P, Dai W, Wang Q, Solinsky R, Chen B, He Z
Nature communications 2021 Feb 3;12(1):781

Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer.

Becker LM, O'Connell JT, Vo AP, Cain MP, Tampe D, Bizarro L, Sugimoto H, McGow AK, Asara JM, Lovisa S, McAndrews KM, Zielinski R, Lorenzi PL, Zeisberg M, Raza S, LeBleu VS, Kalluri R
Cell reports 2020 Jun 2;31(9):107701

PDGF Modulates BMP2-Induced Osteogenesis in Periosteal Progenitor Cells.

Wang X, Matthews BG, Yu J, Novak S, Grcevic D, Sanjay A, Kalajzic I
JBMR plus 2019 May;3(5):e10127

Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.

Gao SY, Zheng GS, Wang L, Liang YJ, Zhang SE, Lao XM, Li K, Liao GQ
PloS one 2017;12(6):e0179248

Osteogenic potential of alpha smooth muscle actin expressing muscle resident progenitor cells.

Matthews BG, Torreggiani E, Roeder E, Matic I, Grcevic D, Kalajzic I
Bone 2016 Mar;84:69-77

Heterogeneous perivascular cell coverage affects breast cancer metastasis and response to chemotherapy.

Kim J, de Sampaio PC, Lundy DM, Peng Q, Evans KW, Sugimoto H, Gagea M, Kienast Y, Amaral NSD, Rocha RM, Eikesdal HP, Lønning PE, Meric-Bernstam F, LeBleu VS
JCI insight 2016 Dec 22;1(21):e90733

Bile acids induce hepatic differentiation of mesenchymal stem cells.

Sawitza I, Kordes C, Götze S, Herebian D, Häussinger D
Scientific reports 2015 Aug 25;5:13320

Hepatic stellate cells support hematopoiesis and are liver-resident mesenchymal stem cells.

Kordes C, Sawitza I, Götze S, Häussinger D
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2013;31(2-3):290-304

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PDGF Receptor beta in extracts from NIH/3T3 and C6 cell lines using PDGF Receptor beta monoclonal antibody (Product # MA5-15143).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PDGF Receptor beta in NIH/3T3 cells, PDGF-treated, using a PDGF Receptor beta monoclonal antibody (Product # MA5-15143) (green). DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PDGF Receptor beta in NIH/3T3 cells, PDGF-treated, using a PDGF Receptor beta monoclonal antibody (Product # MA5-15143) (green). DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of PDGFRB using a monoclonal antibody (Product # MA5-15143).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of PDGF Receptor beta in paraffin-embedded human colon carcinoma using a PDGF Receptor beta monoclonal antibody (Product # MA5-15143).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Periosteal progenitor cells express PDGFRbeta during fracture repair. ( A,B ) Representative images of PDGFR expression in femurs 10 days post fracture. ( A ) Frozen section of fractured femur was stained with Safranin O/fast green. Callus area was magnified in the box. ( B ) Immunostaining of PDGFRbeta and PDGFRalpha on the same area. Boxed areas show higher magnification. (Red: Alexa Fluor 647; Blue: DAPI). Scale bars = 1000 mum. ( C ) Analysis of the frequency of PDGFR + cells in day 10 periosteal callus by flow cytometry. PDGFR expression was analyzed within (CD45, Ter119, CD31) - population. Representative dot plots of flow cytometry are shown. ( D ) Quantification of cell frequency at different time points following fracture. Both tibias from two mice were pooled for each sample, n = 3 samples for each group. ( E ) Representative images of CFU-F assay. Cells were sorted based on PDGFR expression from (CD45/Ter119/CD31) - population of intact periosteum. Colonies were stained with crystal violet. ( F ) Quantification of the number of CFU-F colonies from two independent sorting experiments. ( G ) Representative images of CFU-ALP assay. Sorted cells were induced to osteogenic differentiation for 14 days. Osteoblastic colonies were shown by ALP staining. Values are mean +- SD. * p < 0.05 versus control,** p < 0.01 versus control, # p < 0.01. Copyright (c) 2017 Wiley Periodicals, Inc.

MA5-15143

Antibody data