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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB1263 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PDGF R beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta-subunit complexed with 125I-PDGF-BB (3). It does not recognize the PDGF receptor alpha-subunit. Detects the PDGF receptor beta-subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- PR7212
- Vial size
- 250 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.
Submitted references Spatial and temporal characterization of endometrial mesenchymal stem-like cells activity during the menstrual cycle.
The tyrosine phosphatase SHP-2 controls urokinase-dependent signaling and functions in human vascular smooth muscle cells.
Mesenchymal stem cell abnormalities in patients with multiple myeloma.
Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Efficient induction of oligodendrocytes from human embryonic stem cells.
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Xu S, Chan RWS, Ng EHY, Yeung WSB
Experimental cell research 2017 Jan 1;350(1):184-189
Experimental cell research 2017 Jan 1;350(1):184-189
The tyrosine phosphatase SHP-2 controls urokinase-dependent signaling and functions in human vascular smooth muscle cells.
Kiyan J, Haller H, Dumler I
Experimental cell research 2009 Apr 1;315(6):1029-39
Experimental cell research 2009 Apr 1;315(6):1029-39
Mesenchymal stem cell abnormalities in patients with multiple myeloma.
Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ
Leukemia & lymphoma 2007 Oct;48(10):2032-41
Leukemia & lymphoma 2007 Oct;48(10):2032-41
Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
Zhang M, Veselits M, O'Neill S, Hou P, Reddi AL, Berlin I, Ikeda M, Nash PD, Longnecker R, Band H, Clark MR
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4435-43
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4435-43
Ubiquitinylation of Ig beta dictates the endocytic fate of the B cell antigen receptor.
Zhang M, Veselits M, O'Neill S, Hou P, Reddi AL, Berlin I, Ikeda M, Nash PD, Longnecker R, Band H, Clark MR
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4435-43
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4435-43
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Ball SG, Shuttleworth CA, Kielty CM
The Journal of cell biology 2007 May 7;177(3):489-500
The Journal of cell biology 2007 May 7;177(3):489-500
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Ponte AL, Marais E, Gallay N, Langonné A, Delorme B, Hérault O, Charbord P, Domenech J
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Efficient induction of oligodendrocytes from human embryonic stem cells.
Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW
Stem cells (Dayton, Ohio) 2007 Feb;25(2):419-24
Stem cells (Dayton, Ohio) 2007 Feb;25(2):419-24
PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
Li R, Maminishkis A, Wang FE, Miller SS
Investigative ophthalmology & visual science 2007 Dec;48(12):5722-32
Investigative ophthalmology & visual science 2007 Dec;48(12):5722-32
In vitro cytotoxic effects of imatinib in combination with anticancer drugs in human prostate cancer cell lines.
Kübler HR, van Randenborgh H, Treiber U, Wutzler S, Battistel C, Lehmer A, Wagenpfeil S, Hartung R, Paul R
The Prostate 2005 Jun 1;63(4):385-94
The Prostate 2005 Jun 1;63(4):385-94
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Nagineni CN, Kutty V, Detrick B, Hooks JJ
Journal of cellular physiology 2005 Apr;203(1):35-43
Journal of cellular physiology 2005 Apr;203(1):35-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- PDGF R beta in Human Breast Cancer Tissue. PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PDGF R beta Monoclonal Antibody (Catalog # MAB1263) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
