LS-C353225

antibody from LSBio

Targeting: HDAC5 FLJ90614, KIAA0600, NY-CO-9

Western blot

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

HDAC5 protein structure - LS-C353225 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Immunohistochemical analysis of Histone Deacetylase 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemical analysis of Histone Deacetylase 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: Immunohistochemical analysis of Histone Deacetylase 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.