ALX-804-891-C100

antibody from Enzo Life Sciences

Targeting: AIM2 PYHIN4

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

ALX-804-891-C100

Provider

Enzo Life Sciences

Product name

AIM2 (human) monoclonal antibody (3B10)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3B10

Vial size

100 μg

Storage

0

References

Submitted references

AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC.

Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, Caffrey DR, Latz E, Fitzgerald KA
Nature 2009 Mar 26;458(7237):514-8

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AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA.

Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
Nature 2009 Mar 26;458(7237):509-13

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Biochemical and growth regulatory activities of the HIN-200 family member and putative tumor suppressor protein, AIM2.

Cresswell KS, Clarke CJ, Jackson JT, Darcy PK, Trapani JA, Johnstone RW
Biochemical and biophysical research communications 2005 Jan 14;326(2):417-24

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analyisis of AIM2, mAb (c3B10). HL-60 cells were treated with human IFN_ recombinant protein (570202) at 10 ng/ml for 16 hours. The untreated cells were used as the negative control. Cell lysates from control cells and the hIFN_ treated cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with AIM2, mAb (c3B10). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.