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LearnPA5-23182
antibody from Invitrogen Antibodies
Targeting: GPBAR1
BG37, GPCR, GPCR19, GPR131, M-BAR, MGC40597, TGR5
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-23182 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGR5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Microbial bile acid metabolites modulate gut RORγ(+) regulatory T cell homeostasis.
Song X, Sun X, Oh SF, Wu M, Zhang Y, Zheng W, Geva-Zatorsky N, Jupp R, Mathis D, Benoist C, Kasper DL
Nature 2020 Jan;577(7790):410-415
Nature 2020 Jan;577(7790):410-415
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TGR5 in spleen lysate. Samples were incubated in TGR5 polyclonal antibody (Product # PA5-23182). (1) Pre-immune sera. TGR5/GPBAR1 antibody tested on (2) Human 1:1000, (3) Mouse 1:5000, and (4) Rat 1:5000 spleen lysates.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TGR5 in Tissue section of mouse colon. Samples were incubated in TGR5 polyclonal antibody (Product # PA5-23182) using a dilution of 1:200. The signal was detected using HRP-labelled secondary antibody and DAB staining which followed counterstaining with hematoxylin. This TGR5/GPBAR1 antibody generated a very specific a cytoplasmic staining primarily in the columner epithelial cells. A subset of cells from mucosa muscularis and the lamina propria also showed TGR5 positivity. Some cells in all histological layers depicted a nuclear staining also.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
