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LearnMAB4286
antibody from R&D Systems
Targeting: GPBAR1
BG37, GPCR, GPCR19, GPR131, M-BAR, MGC40597, TGR5
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- MAB4286 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TGR5/GPBAR1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human TGR5/GPBAR1 transfectants but not irrelevant transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8TDU6- Isotype
- IgG
- Antibody clone number
- 409522
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TGR5 signalling inhibits the production of pro-inflammatory cytokines by in vitro differentiated inflammatory and intestinal macrophages in Crohn's disease.
Yoneno K, Hisamatsu T, Shimamura K, Kamada N, Ichikawa R, Kitazume MT, Mori M, Uo M, Namikawa Y, Matsuoka K, Sato T, Koganei K, Sugita A, Kanai T, Hibi T
Immunology 2013 May;139(1):19-29
Immunology 2013 May;139(1):19-29
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TGR5/GPBAR1 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TGR5/GPBAR1 Mono-clonal Antibody (Catalog # MAB4286, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TGR5/GPBAR1 in HEK293 Human Cell Line transfected with TGR5/GPBAR1 and EGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human TGR5/GPBAR1 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human TGR5/GPBAR1 Monoclonal Antibody (Catalog # MAB4286) followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on Mouse IgG2B Isotype Control (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
