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- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-17071 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF4B Monoclonal Antibody (1F5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17071 targets EIF4B in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples. The MA5-17071 immunogen is purified recombinant fragment of human EIF4B expressed in E. Coli. MA5-17071 detects EIF4B which has a predicted molecular weight of approximately 80kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B using a EIF4B monoclonal antibody (Product # MA5-17071) against a human EIF4B (AA: 381-585) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B using EIF4B monoclonal antibody (Product # MA5-17071) in A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B using a EIF4B monoclonal antibody (Product # MA5-17071) against a human EIF4B (AA: 381-585) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B using EIF4B monoclonal antibody (Product # MA5-17071) in A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH3T3 cells using EIF4B monoclonal antibody (Product # MA5-17071) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B monoclonal antibody (Product # MA5-17071) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using EIF4B monoclonal antibody (Product # MA5-17071) (green) and negative control (red).
