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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51400PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51400PU-N, RRID:AB_11149654
- Product name
- anti EIF4B (Center)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the Central region of Human EIF4B
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (pS498) (Cat. #AP51400PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4B gene.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4B Antibody Cat.-No AP51400PU-N in MCF-7 cell line lysates (35µg/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma reacted with EIF4B Antibody Cat.-No AP51400PU-N peroxidase conjugated to the secondary antibody and followed by DAB staining.This data demonstrates the use of EIF4B Antibody (Center S498) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using EIF4B Antibody Cat.-No AP51400PU-N (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
