Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM06769PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM06769PU-N, RRID:AB_11217163
- Product name
- anti EIF4B
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment of Human EIF4B expressed in E. Coli.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F5
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using EIF4B antibody Cat.-No AM06769PU-N against A549 (Lane 1), A431 (Lane 2), HepG2 (Lane 3), HEK293 (Lane 4), HeLa (Lane 5), Jurkat (Lane 6), K562 (Lane 7), NIH3T3 (Lane 8), and MCF-7 (Lane 9) cell lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using EIF4B antibody Cat.-No AM06769PU-N against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Red: Control Antigen (100 ng)Purple: Antigen (10 ng)Green: Antigen (50 ng)Blue: Antigen (100 ng)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH3T3 cells using EIF4B antibbody Cat.-No AM06769PU-N EIF4B (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using EIF4B antibody Cat.-No AM06769PU-N (green) and Negative Control (red).