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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-47191 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP27A1 Recombinant Rabbit Monoclonal Antibody (HL1820)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- HL1820
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP27A1 in Various tissue extracts (50 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with CYP27A1 Monoclonal antibody (Product # MA5-47191) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP27A1 in Various whole cell extracts (30 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with CYP27A1 Monoclonal antibody (Product # MA5-47191) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CYP27A1 in HepG2 cells. Cells were fixed in ice-cold MeOH for 5 min. Green: CYP27A1 stained by CYP27A1 monoclonal antibody (Product # MA5-47191) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP27A1 in paraffin-embedded mouse kidney. Samples were incubated in CYP27A1 Monoclonal antibody (Product # MA5-47191). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP27A1 in paraffin-embedded rat kidney. Samples were incubated in CYP27A1 Monoclonal antibody (Product # MA5-47191). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
