Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-47191 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP27A1 Monoclonal Antibody (HL1820)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Store as concentrated solution.
- Antibody clone number
- HL1820
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP27A1 in Various tissue extracts (50 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with CYP27A1 Monoclonal antibody (Product # MA5-47191) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP27A1 in Various whole cell extracts (30 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with CYP27A1 Monoclonal antibody (Product # MA5-47191) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CYP27A1 in HepG2 cells. Cells were fixed in ice-cold MeOH for 5 min. Green: CYP27A1 stained by CYP27A1 monoclonal antibody (Product # MA5-47191) diluted at 1:500. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP27A1 in paraffin-embedded mouse kidney. Samples were incubated in CYP27A1 Monoclonal antibody (Product # MA5-47191). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP27A1 in paraffin-embedded rat kidney. Samples were incubated in CYP27A1 Monoclonal antibody (Product # MA5-47191). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
