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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1936 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1936, RRID:AB_1581776
- Product name
- USP21 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of USP21.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human and mouse proteases: a comparative genomic approach.
Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins.
Generation and analysis of 280,000 human expressed sequence tags.
Puente XS, Sánchez LM, Overall CM, López-Otín C
Nature reviews. Genetics 2003 Jul;4(7):544-58
Nature reviews. Genetics 2003 Jul;4(7):544-58
Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins.
Gong L, Kamitani T, Millas S, Yeh ET
The Journal of biological chemistry 2000 May 12;275(19):14212-6
The Journal of biological chemistry 2000 May 12;275(19):14212-6
Generation and analysis of 280,000 human expressed sequence tags.
Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M
Genome research 1996 Sep;6(9):807-28
Genome research 1996 Sep;6(9):807-28
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of USP21 polyclonal antibody (Cat # PAB1936) in T-47D cell line lysates (35 ug/lane). USP21 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with USP21 polyclonal antibody (Cat # PAB1936) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
