Antibody data
- Product number
- MA5-34953
- Provider
- Invitrogen Antibodies
- Product name
- USP21 Monoclonal Antibody (16E1)
- Provider product page
- Invitrogen Antibodies - MA5-34953
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 16E1
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP21 in Positive control: Lane 1: SH-SY5Y cell lysates, Lane 2: K562 cell lysates. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with USP21 monoclonal antibody (Product # MA5-34953), at a dilution of 1:500, followed by Goat Anti-Mouse IgG - HRP (1 hour) with a dilution of 1:5000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP21 in Positive control: Lane 1: SH-SY5Y cell lysates, Lane 2: K562 cell lysates. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with USP21 monoclonal antibody (Product # MA5-34953), at a dilution of 1:500, followed by Goat Anti-Mouse IgG - HRP (1 hour) with a dilution of 1:5000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of USP21 in 293T cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of USP21 in 293T cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:1000.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP21 in paraffin-embedded human small intestine tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP21 in paraffin-embedded rat seminal vesicle tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP21 in paraffin-embedded mouse heart tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP21 in paraffin-embedded human liver tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of USP21 in paraffin-embedded human kidney tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of USP21 in SH-SY5Y cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated Goat anti-Mouse IgG with a dilution of 1:1000 (30 min).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of USP21 in SH-SY5Y cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with USP21 monoclonal antibody (Product # MA5-34953) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated Goat anti-Mouse IgG with a dilution of 1:1000 (30 min).
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