Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-112665 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPOX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 5 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of PPOX in multiple sample types using a PPOX polyclonal antibody (Product # PA5-112665) at a concentration of 2.5 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: A549 whole cell lysate, HepG2 whole cell lysate .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of PPOX in PC-3 cells using a Polyclonal antibody (Product # PA5-112665) at a dilution of 1:100. An Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibdoy was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of paraffin-embeded PPOX in human testis tissue using a PPOX Polyclonal antibody (Product # PA5-112665) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of PPOX in HepG2 whole cell lysate using a Polyclonal antibody (Product #PA5-112665). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 1: Rabbit control IgG, Lane 2: 8 µg of primary antibody and 500 µg of HepG2 whole cell lysate, Lane 3: 10 µg of HepG2 whole cell lysate.