AF800

antibody from R&D Systems

Targeting: BCL2L1 Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AF800

Provider

R&D Systems

Product name

Human/Mouse Bcl-x Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and mouse Bcl-x in Western blots.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint.

Schmitt E, Beauchemin M, Bertrand R
Oncogene 2007 Aug 30;26(40):5851-65

---

Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.

Armstrong T, Packham G, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Nov 1;10(21):7427-37

---

Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.

Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8

---

Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.

Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8

---

The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.

Sade H, Krishna S, Sarin A
The Journal of biological chemistry 2004 Jan 23;279(4):2937-44

---

Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.

Raman VS, Akondy RS, Rath S, Bal V, George A
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81

---

Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.

Raman VS, Akondy RS, Rath S, Bal V, George A
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81

---

T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.

Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
The Journal of experimental medicine 2002 Jul 1;196(1):87-95

---

T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.

Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
The Journal of experimental medicine 2002 Jul 1;196(1):87-95

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse Bcl-x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 10⁴ cells, lane 1 and 2.5 x 10⁴ cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse Bcl-x by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl-x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunoprecipitation

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Immunoprecipitation of Human Bcl-x. Bcl-x was immunoprecipitated from lysates (7 x 10⁵ cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30-60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.