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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB7119 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7119, RRID:AB_1578040
- Product name
- NHEJ1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of NHEJ1.
- Antigen sequence
C-QRPQLSKVKRKKPR- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining.
Ahnesorg P, Smith P, Jackson SP
Cell 2006 Jan 27;124(2):301-13
Cell 2006 Jan 27;124(2):301-13
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NHEJ1 polyclonal antibody (Cat # PAB7119) (0.1 ug/mL) staining of human thyroid lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NHEJ1 polyclonal antibody (Cat # PAB7119) (3.8 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
