NBP2-03275

antibody from Novus Biologicals

Targeting: NHEJ1 Cernunnos, FLJ12610, XLF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-03275

Provider

Novus Biologicals

Product name

Mouse Monoclonal XLF Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.52 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

XLF and APLF bind Ku80 at two remote sites to ensure DNA repair by non-homologous end joining.

Nemoz C, Ropars V, Frit P, Gontier A, Drevet P, Yu J, Guerois R, Pitois A, Comte A, Delteil C, Barboule N, Legrand P, Baconnais S, Yin Y, Tadi S, Barbet-Massin E, Berger I, Le Cam E, Modesti M, Rothenberg E, Calsou P, Charbonnier JB
Nature structural & molecular biology 2018 Oct;25(10):971-980

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Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair.

Reid DA, Keegan S, Leo-Macias A, Watanabe G, Strande NT, Chang HH, Oksuz BA, Fenyo D, Lieber MR, Ramsden DA, Rothenberg E
Proceedings of the National Academy of Sciences of the United States of America 2015 May 19;112(20):E2575-84

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: XLF Antibody (3D6) [NBP2-03275] Analysis of extracts (35ug) from 9 different cell lines by using anti-XLF monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: XLF Antibody (3D6) [NBP2-03275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XLF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XLF.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human endometrium tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human lymph node tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human prostate tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human lymphoma tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human Kidney tissue using anti-XLF mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human liver tissue using anti-XLF mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: XLF Antibody (3D6) [NBP2-03275] - Analysis of Hela cells, using anti-XLF antibody, (Red), compared to a nonspecific negative control antibody (Blue).