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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324637 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ATG9A Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ATG9A Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATG9A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.4 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-Autophagy APG9L1 Antibody (C-term) (Cat.#TA324637) in A2058 and A375 cell line lysates (35ug/lane). APG9L1(arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cells stained with ATG9A (C-term) antibody. U251 cells were treated with Chloroquine , then incubated with TA324637 ATG9A (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC
