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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- F54719 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ATG9A Antibody
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 µl, 0.4 µl
- Storage
- Aliquot the ATG9A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A375 and 2) HepG2 cell lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A2058 (left) and A375 (right) cell lysate with ATG9A antibody. Predicted molecular weight ~94 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human U-251 cells (treated with 50 uM Chloroquine for 16 hr) with ATG9A antibody (green) and Hoechst 33342 nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer tissue with ATG9A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
