GTX101254

antibody from GeneTex

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX101254

Provider

GeneTex

Proper citation

GeneTex Cat#GTX101254, RRID:AB_1950064

Product name

beta Catenin antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

MiRNA-21 induces epithelial to mesenchymal transition and gemcitabine resistance via the PTEN/AKT pathway in breast cancer.

Wu ZH, Tao ZH, Zhang J, Li T, Ni C, Xie J, Zhang JF, Hu XC
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jun;37(6):7245-54

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Starvation beneficially influences the liver physiology and nutrient metabolism in Edwardsiella tarda infected red sea bream (Pagrus major).

Mohapatra S, Chakraborty T, Shimizu S, Urasaki S, Matsubara T, Nagahama Y, Ohta K
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2015 Nov;189:1-10

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Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors.

Król M, Mucha J, Majchrzak K, Homa A, Bulkowska M, Majewska A, Gajewska M, Pietrzak M, Perszko M, Romanowska K, Pawłowski K, Manuali E, Hellmen E, Motyl T
PloS one 2014;9(1):e83995

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Arsenic exposure inhibits myogenesis and neurogenesis in P19 stem cells through repression of the β-catenin signaling pathway.

Hong GM, Bain LJ
Toxicological sciences : an official journal of the Society of Toxicology 2012 Sep;129(1):146-56

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX101254) diluted at 1:1000.

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

39918

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE GTX101254 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX101254 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX101254) diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Catenin antibody detects CTNNB1 protein at membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. beta Catenin antibody (GTX101254) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Catenin antibody detects beta Catenin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody (GTX101254) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Catenin antibody detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. beta Catenin antibody (GTX101254) dilution: 1:500.