Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB13291-050 - Provider product page
- Provider
- R&D Systems
- Product name
- Human beta-Catenin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human beta-Catenin in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 196618
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tissue expression of β-catenin and E- and N-cadherins in chronic hepatitis C and hepatocellular carcinoma.
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells.
E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
Survivin in the human hair follicle.
Kasprzak A, Rogacki K, Adamek A, Sterzyńska K, Przybyszewska W, Seraszek-Jaros A, Helak-Łapaj C, Pyda P
Archives of medical science : AMS 2017 Oct;13(6):1269-1280
Archives of medical science : AMS 2017 Oct;13(6):1269-1280
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Riordan DP, Varma S, West RB, Brown PO
PloS one 2015;10(7):e0128975
PloS one 2015;10(7):e0128975
SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells.
Vicente CM, Lima MA, Nader HB, Toma L
Journal of experimental & clinical cancer research : CR 2015 Mar 14;34:25
Journal of experimental & clinical cancer research : CR 2015 Mar 14;34:25
E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
Moore RN, Cherry JF, Mathur V, Cohen R, Grumet M, Moghe PV
Stem cells and development 2012 Jan;21(1):30-41
Stem cells and development 2012 Jan;21(1):30-41
Survivin in the human hair follicle.
Botchkareva NV, Kahn M, Ahluwalia G, Shander D
The Journal of investigative dermatology 2007 Feb;127(2):479-82
The Journal of investigative dermatology 2007 Feb;127(2):479-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- beta-Catenin in SW480 Human Cell Line. beta-Catenin was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using 10 µg/mL Mouse Anti-Human beta-Catenin Monoclonal Antibody (Catalog # MAB13291) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- beta-Catenin in Human Pancreas. beta-Catenin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human beta-Catenin Monoclonal Antibody (Catalog # MAB13291) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.