STJ11101750

antibody from St John's Laboratory

Targeting: CTNNB1 armadillo, beta-catenin, CTNNB

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: STJ11101750 - Provider product page
Provider: St John's Laboratory
Product name: Anti-CTNNB1 antibody [ARC0136] (STJ11101750)
Antibody type: Monoclonal
Description: Rabbit monoclonal antibody anti-Beta-Catenin is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Handling: NA

CTNNB1 protein structure - STJ11101750 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of HeLa cells, using Beta-Catenin antibody (STJ11101750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts of mouse brain, using Beta-Catenin antibody (STJ11101750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Western blot analysis of extracts from normal (control) and Beta-Catenin knockout (KO) 293T cells, using Beta-Catenin antibody (STJ11101750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunofluorescence analysis of C6 cells using Beta-Catenin antibody (STJ11101750) at dilution of 1:100. Blue: DAPI for nuclear staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded Human cervical squamous cell carcinoma using [KO Validated] Beta-Catenin rabbit monoclonal antibody (STJ11101750) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded Human Invasive ductal carcinoma of breast using [KO Validated] Beta-Catenin rabbit monoclonal antibody (STJ11101750) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using [KO Validated] Beta-Catenin rabbit monoclonal antibody (STJ11101750) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded Human pancreatic ductal carcinoma using [KO Validated] Beta-Catenin rabbit monoclonal antibody (STJ11101750) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA