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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- TA501720 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501720, RRID:AB_11124551
- Product name
- UHMK1 (KIST) mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)
- Antibody type
- Monoclonal
- Description
- UHMK1 (KIST) mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- UHMK1
- Isotype
- IgG
- Antibody clone number
- OTI2H4
- Vial size
- 100 µl
- Concentration
- 0.82 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UHMK1 (RC214962, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UHMK1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UHMK1 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-UHMK1 mouse monoclonal antibody (TA501720) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UHMK1(RC214962).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UHMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501720)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UHMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501720)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-UHMK1 antibody(TA501720),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
