Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 15016-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNPEP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: FDRDLTLAG RVIVKCPTSG RLEQQLVHVE RPILRIPHLA IHLQRNINEN FGPNTEMHLV PILATAIQEE LEKGTPEPGP LNAVDERHHS VLMSLLCAHL GLSPKDIVEM ELCLADTQPA VLGGAYDEFI FAPRLDNLHS CFCALQALID SCAGPGSLAT EPHVRMVTLY DNEEVGSESA QGAQSLLTEL VLRRISASCQ HPTAFEEAIP KSFMISADMA HAVHPNYLDK HEENHRPLFH KGPVIKVNSK QRYASNAVSE ALIREVANKV KVPLQDLMVR NDTPCGTTIG PILASRLGLR VLDLGSPQLA MHSIREMACT TGVLQTLTLF KGFFELFPSL SHNLLVD (130-475 aa encoded by BC000653 )
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 15016-1-AP (DNPEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human spleen tissue were subjected to SDS PAGE followed by western blot with 15016-1-AP (DNPEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using 15016-1-AP (DNPEP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DNPEP (IP:15016-1-AP, 3ug; Detection:15016-1-AP 1:500) with HEK-293 cells lysate 1760ug.