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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2754 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2754, RRID:AB_1581691
- Product name
- UCK2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of UCK2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A crucial role of uridine/cytidine kinase 2 in antitumor activity of 3'-ethynyl nucleosides.
Reaction of human UMP-CMP kinase with natural and analog substrates.
Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates.
Murata D, Endo Y, Obata T, Sakamoto K, Syouji Y, Kadohira M, Matsuda A, Sasaki T
Drug metabolism and disposition: the biological fate of chemicals 2004 Oct;32(10):1178-82
Drug metabolism and disposition: the biological fate of chemicals 2004 Oct;32(10):1178-82
Reaction of human UMP-CMP kinase with natural and analog substrates.
Pasti C, Gallois-Montbrun S, Munier-Lehmann H, Veron M, Gilles AM, Deville-Bonne D
European journal of biochemistry / FEBS 2003 Apr;270(8):1784-90
European journal of biochemistry / FEBS 2003 Apr;270(8):1784-90
Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates.
Liou JY, Dutschman GE, Lam W, Jiang Z, Cheng YC
Cancer research 2002 Mar 15;62(6):1624-31
Cancer research 2002 Mar 15;62(6):1624-31
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of UCK2 polyclonal antibody (Cat # PAB2754) pre-incubated with and without blocking peptide (BP7192a) in HeLa cell line lysate. UCK2 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with UCK2 polyclonal antibody (Cat # PAB2754) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
