A300-663A

antibody from Invitrogen Antibodies

Targeting: TIPRL dJ69E11.3, MGC3794, TIP41, TIPRL1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-663A

Provider

Invitrogen Antibodies

Product name

Tip41 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, A-549, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (Product # A300-663A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse Tip41 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (Product # A300-663A lot 2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Tip41 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP; 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Tip41 antibody BL3057 (Cat. No. A300-663A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Efficiency of immunoprecipitation of Tip41 was much less when using rabbit anti-Tip41 antibody BL3056 (Cat. No. A300-662A) or BL3058. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Tip41 (Product # A300-663A; lot 2) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Tip41 (Cat. No. A300-663A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Tip41 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Tip41 antibody (Product # A300-663A lot 2) used for IP at 6 µg per reaction. Tip41 was also immunoprecipitated by a previous lot of this antibody (A300-663A lot 1). For blotting immunoprecipitated Tip41 (Product # A300-663A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.