LS-C100259

antibody from LSBio

Targeting: EPHA4 Hek8, TYRO1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C100259

Provider

LSBio

Product name

EPHA4 / EPH Receptor A4 Antibody (aa40-70) LS-C100259

Antibody type

Polyclonal

Description

Ammonium sulfate precipitation

Reactivity

Human

Host

Rabbit

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot of EphA4 (arrow) using EphA4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA4 gene (Lane 2) (Origene Technologies).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot of anti-EphA4 N-term antibody in HeLa cell lysate. EphA4 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.