AF641

antibody from R&D Systems

Targeting: EPHA4 Hek8, TYRO1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF641

Provider

R&D Systems

Product name

Mouse EphA4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse EphA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human EphA4 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA6, rmEphA7, and rmEphA8 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q03137


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse.

Collinson JM, Lindström NO, Neves C, Wallace K, Meharg C, Charles RH, Ross ZK, Fraser AM, Mbogo I, Oras K, Nakamoto M, Barker S, Duce S, Miedzybrodzka Z, Vargesson N
Development (Cambridge, England) 2018 Feb 8;145(3)

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EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche.

Todd KL, Baker KL, Eastman MB, Kolling FW, Trausch AG, Nelson CE, Conover JC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Mar 22;37(12):3331-3341

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α2-chimaerin is required for Eph receptor-class-specific spinal motor axon guidance and coordinate activation of antagonistic muscles.

Kao TJ, Nicholl GC, Johansen JA, Kania A, Beg AA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 11;35(6):2344-57

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Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear.

Raft S, Andrade LR, Shao D, Akiyama H, Henkemeyer M, Wu DK
Developmental biology 2014 Jun 1;390(1):51-67

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A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.

Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
European journal of immunology 2012 Jun;42(6):1562-72

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Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.

Kuwako K, Kakumoto K, Imai T, Igarashi M, Hamakubo T, Sakakibara S, Tessier-Lavigne M, Okano HJ, Okano H
Neuron 2010 Aug 12;67(3):407-21

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Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.

Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB
Nature neuroscience 2003 Feb;6(2):153-60

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

EphA4 in Embryonic Rat Rib Cartilage Primordium. EphA4 was detected in immersion fixed frozen sections of embryonic rat rib cartilage primordium (E15) using 5 µg/mL Goat Anti-Mouse EphA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF641) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.