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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3007 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3007, RRID:AB_1574704
- Product name
- EPHA4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EPHA4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Eph/ephrin profiling in human breast cancer reveals significant associations between expression level and clinical outcome.
Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred.
Roles of Eph receptors and ephrins in segmental patterning.
Eph receptors and ephrins: effectors of morphogenesis.
Brantley-Sieders DM, Jiang A, Sarma K, Badu-Nkansah A, Walter DL, Shyr Y, Chen J
PloS one 2011;6(9):e24426
PloS one 2011;6(9):e24426
Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred.
Prevost N, Woulfe D, Tanaka T, Brass LF
Proceedings of the National Academy of Sciences of the United States of America 2002 Jul 9;99(14):9219-24
Proceedings of the National Academy of Sciences of the United States of America 2002 Jul 9;99(14):9219-24
Roles of Eph receptors and ephrins in segmental patterning.
Xu Q, Mellitzer G, Wilkinson DG
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2000 Jul 29;355(1399):993-1002
Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2000 Jul 29;355(1399):993-1002
Eph receptors and ephrins: effectors of morphogenesis.
Holder N, Klein R
Development (Cambridge, England) 1999 May;126(10):2033-44
Development (Cambridge, England) 1999 May;126(10):2033-44
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EPHA4 polyclonal antibody (Cat # PAB3007) in HeLa cell lysate. EPHA4 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EPHA4 (arrow) using EPHA4 polyclonal antibody (Cat # PAB3007).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Methanol/Acetone fixed human stem cell is used in IF to detect EPHA4 (blue) and endothelial Lectin (red) . Data kindly provided by Dr. Weis from Cheresh Lab, UCSD.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA4 polyclonal antibody (Cat # PAB3007) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CEM cells using EPHA4 polyclonal antibody (Cat # PAB3007)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
