F54444

antibody from NSJ Bioreagents

Targeting: EPHA4 Hek8, TYRO1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54444

Provider

NSJ Bioreagents

Product name

EphA4 Antibody

Antibody type

Polyclonal

Description

SAS precipitation

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the EphA4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa and 2) NCI-H460 cell lysate with EphA4 antibody. Expected molecular weight: 110-130 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with EphA4 antibody.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with EphA4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human CCRF-CEM cells with EphA4 antibody; Blue=isotype control, Green= EphA4 antibody.