21875-1-AP

antibody from Invitrogen Antibodies

Targeting: EPHA4 Hek8, TYRO1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21875-1-AP

Provider

Invitrogen Antibodies

Product name

EPHA4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KDQNERSYR IVRTAARNTD IKGLNPLTSY VFHVRARTAA GYGDFSEPLE VTTNTVPSRI IGDGANSTVL LVSVSGSVVL VVILIAAFVI SRRRSKYSKA KQEADEEKHL NQGVRTYVDP FTYE (481-603 aa encoded by B C026327)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.46 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21875-1-AP (EPHA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result of EPHA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 SH-SY5Y cells were stained with 0.2ug EPHA4 antibody (21875-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EPHA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug.