PAB22020

antibody from Abnova Corporation

Targeting: CCDC181 C1orf114, FLJ25846

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22020

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22020, RRID:AB_10962809

Product name

C1orf114 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant C1orf114.

Antigen sequence

KSNHRTQSAHISPVTSTYCLSPRQKELQKQLEEKR
EKLKREEERRKIEEEKEKKRENDIVFKAWLQKKRE
QVLEMRRIQRAK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with C1orf114 polyclonal antibody (Cat # PAB22020) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with C1orf114 polyclonal antibody (Cat # PAB22020) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human nasopharynx with C1orf114 polyclonal antibody (Cat # PAB22020) shows strong membranous positivity in respiratory epithelial cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)