Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX112975 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112975, RRID:AB_1950655
- Product name
- Cytokeratin 8 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.
Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Yu HI, Chou HC, Su YC, Lin LH, Lu CH, Chuang HH, Tsai YT, Liao EC, Wei YS, Yang YT, Lee YR, Chan HL
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350
Journal of pharmaceutical and biomedical analysis 2018 Oct 25;160:344-350
Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Li JM, Chou HC, Wang SH, Wu CL, Chen YW, Lin ST, Chen YH, Chan HL
Environmental and molecular mutagenesis 2013 Jul;54(6):429-49
Environmental and molecular mutagenesis 2013 Jul;54(6):429-49
Proteomic analysis of retinopathy-related plasma biomarkers in diabetic patients.
Lu CH, Lin ST, Chou HC, Lee YR, Chan HL
Archives of biochemistry and biophysics 2013 Jan 15;529(2):146-56
Archives of biochemistry and biophysics 2013 Jan 15;529(2):146-56
Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.
Cho CK, Smith CR, Diamandis EP
Journal of proteome research 2010 Jul 2;9(7):3574-82
Journal of proteome research 2010 Jul 2;9(7):3574-82
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse liver 10% SDS PAGE GTX112975 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE GTX112975 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cytokeratin 8 antibody [N1C1] detects Cytokeratin 8 protein by western blot analysis.A. 30 ?g H1299 whole cell lysate/extract10% SDS-PAGECytokeratin 8 antibody [N1C1] (GTX112975) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] (GTX112975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8(GTX112975) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8(GTX112975) antibody at 1:500 dilution.