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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- UM500001 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500001, RRID:AB_2629017
- Product name
- KRT8/CK8 mouse monoclonal antibody, clone UMAB1
- Antibody type
- Monoclonal
- Description
- KRT8/CK8 mouse monoclonal antibody, clone UMAB1
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KRT8
- Isotype
- IgG
- Antibody clone number
- UMAB1
- Vial size
- 100 µl
- Concentration
- 0.5~1.0 mg/ml (Lot Dependent)
Submitted references An isolated orbital mass with proptosis and diplopia: an uncommon metastasis from intracranial olfactory neuroblastoma in a chinese patient.
Shen J, Cui H, Feng S
OncoTargets and therapy 2016;9:5281-6
OncoTargets and therapy 2016;9:5281-6
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (Clone UMAB1) at 1:500.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of mouse tissue lysates (20ug) from Colon. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot? Ultra (1:1000).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using anti-CK8 mouse monoclonal antibody (UM500001, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-KRT8mouse monoclonal antibody. (Clone UMAB1, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT8 mouse monoclonal antibody (Clone UMAB1). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-KRT8 (Clone UMAB1) very specifically recognizes KRT8 antigen on OriGene protein microarray chip.
- Validation comment
- 10K-CHIP
