M01421-2

antibody from Boster Biological Technology

Targeting: KRT8 CARD2, CK8, CYK8, K2C8, K8, KO

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M01421-2

Provider

Boster Biological Technology

Product name

Anti-Cytokeratin 8 KRT8 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Cytokeratin 8/KRT8 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse Cytokeratin 8/KRT8 information: Molecular Weight: 53704 MW; Subcellular Localization: Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix ; Tissue Specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

Reactivity

Human, Mouse

Host

Rabbit

Antibody clone number

CIG-11

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver, using Cytokeratin 8 Antibody(M01421-2)KRT8 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KRT8 Antibody (M01421-2)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image