M01421-3

antibody from Boster Biological Technology

Targeting: KRT8 CARD2, CK8, CYK8, K2C8, K8, KO

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

M01421-3

Provider

Boster Biological Technology

Product name

Anti-Cytokeratin 8 KRT8 Antibody Picoband™ (monoclonal, 3G9)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for Cytokeratin 8 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3G9

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8 antibody (M01421-3). Overlay histogram showing A549 cells stained with M01421-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 8 Antibody (M01421-3, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image