Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2537 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Myocilin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Myocilin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q99972
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle.
Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells.
Reduced myocilin expression in cultured monkey trabecular meshwork cells induced by a selective glucocorticoid receptor agonist: comparison with steroids.
Substrate rigidity modulates cell matrix interactions and protein expression in human trabecular meshwork cells.
Lynch JM, Dolman AJ, Guo C, Dolan K, Xiang C, Reda S, Li B, Prasanna G
PloS one 2018;13(11):e0206801
PloS one 2018;13(11):e0206801
Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells.
Lynch JM, Li B, Katoli P, Xiang C, Leehy B, Rangaswamy N, Saenz-Vash V, Wang YK, Lei H, Nicholson TB, Meredith E, Rice DS, Prasanna G, Chen A
The Journal of biological chemistry 2018 Dec 28;293(52):20137-20156
The Journal of biological chemistry 2018 Dec 28;293(52):20137-20156
Reduced myocilin expression in cultured monkey trabecular meshwork cells induced by a selective glucocorticoid receptor agonist: comparison with steroids.
Pfeffer BA, DeWitt CA, Salvador-Silva M, Cavet ME, López FJ, Ward KW
Investigative ophthalmology & visual science 2010 Jan;51(1):437-46
Investigative ophthalmology & visual science 2010 Jan;51(1):437-46
Substrate rigidity modulates cell matrix interactions and protein expression in human trabecular meshwork cells.
Schlunck G, Han H, Wecker T, Kampik D, Meyer-ter-Vehn T, Grehn F
Investigative ophthalmology & visual science 2008 Jan;49(1):262-9
Investigative ophthalmology & visual science 2008 Jan;49(1):262-9
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Myocilin by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Myocilin at approximately 55 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Myocilin by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Myocilin at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Myocilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2537) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.