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Western blot
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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-138-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-138-C100, RRID:AB_2051006
- Product name
- CD134L (mouse) monoclonal antibody (blocking) (MGP34)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- MGP34
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Impairment of antigen-presenting cell function in mice lacking expression of OX40 ligand.
Murata K, Ishii N, Takano H, Miura S, Ndhlovu LC, Nose M, Noda T, Sugamura K
The Journal of experimental medicine 2000 Jan 17;191(2):365-74
The Journal of experimental medicine 2000 Jan 17;191(2):365-74
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Flow cytometry using MAb to CD134L (mouse) (blocking) (MGP34) (Prod. No. ALX-804-138). Method: Rat TART-gp34 cells (5x10E5) expressing surface mouse OX40L vector (thick line) or TART-1 parental cells (dotted line), were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to CD134L (mouse) (blocking) (MGP34). After washing in FACS buffer, FITC-conjugated antibody to rat IgG (Prod. No. ALX-211-051) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
