Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-21796 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANKRD45 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 1 mg/mL
Submitted references Ankrd45 Is a Novel Ankyrin Repeat Protein Required for Cell Proliferation.
Kang Y, Xie H, Zhao C
Genes 2019 Jun 16;10(6)
Genes 2019 Jun 16;10(6)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ANKRD45 Polyclonal Antibody (Product # PA5-21796). Sample (30 µg whole cell lysate). A: Hep G2. 12% SDS PAGE. ANKRD45 Polyclonal Antibody (Product # PA5-21796) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ANKRD45 Polyclonal Antibody detects ANKRD45 protein at cytosol on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded normal liver. ANKRD45 Polyclonal Antibody (Product # PA5-21796) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Subcellular localization of ANKRD45 during mitosis. ( A - D ) Subcellular localization of ANKRD45 during mitosis in human hepatoma Hep3B cells. The distribution of ANKRD45 was visualized with anti-ANKRD45 antibody (green). The cytoskeleton was stained by anti-alpha-tubulin antibody (red) and nuclei were labeled with DAPI (blue). ( E ) Hep3B cells were treated with control or four siRNAs against ANKRD45 and analyzed with Western blotting using ANKRD45 antibody or a cleaved form of the PARP antibody. The alpha-tubulin antibody was used as a loading control. Bottom bar graph shows the statistical results of ANKRD45 expression in control or siRNA-treated cells from the immunoblots results. ( F , G ) Confocal images showing the localization of ANKRD45 in the midbody during cytokinesis in control ( F ) or siRNA#2 ( G ) treated HeLa cells. ( H ) Quantitative analysis of relative ANKRD45 expression in the midbody ring of control or siRNA#2-treated HeLa cells. NC, negative control. Scale bars: 5 mum. *** p < 0.001.