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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-57840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIP12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AMCKEIIPTS EFINSKLTAK ANRQLQDPLV IMTGNIPTWL TELGKTCPFF FPFDTRQMLF YVTAFDRDRA MQRLLDTNPE INQSDSQD Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Helicobacter pylori pathogen inhibits cellular responses to oncogenic stress and apoptosis.
Palrasu M, Zaika E, Paulrasu K, Caspa Gokulan R, Suarez G, Que J, El-Rifai W, Peek RM Jr, Garcia-Buitrago M, Zaika AI
PLoS pathogens 2022 Jun;18(6):e1010628
PLoS pathogens 2022 Jun;18(6):e1010628
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TRIP12 in human cell line U-2 OS using a TRIP12 Polyclonal Antibody (Product # PA5-57840) shows localization to nuclear speckles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TRIP12 in human testis using TRIP12 Polyclonal Antibody (Product # PA5-57840) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TRIP12 in human pancreas using TRIP12 Polyclonal Antibody (Product # PA5-57840) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.ppat.1010628.g005 Fig 5 Analyses of ULF and SIVA1 protein expression in the murine stomach. (A) A representative image showing IHC staining for SIVA1 protein in the corpus and antrum of H . pylori -infected and non-infected mice (n = 6/group). (B) The same as (A) but ULF protein staining is shown. (C) The same as (A and B), but Western blotting was used for analyses of ULF and SIVA1 proteins in gastric tissue homogenates. Bottom panel shows the densitometric analyses. (D) Protein expression of ULF (left panel) and SIVA1 (right panel) in gastric organoids derived from the antropyloric region of the murine stomach that were infected with H . pylori strain 7.13 in vitro or left uninfected. Panels show representative light microscopic and immunofluorescence images. Bottom panel shows quantification of ULF and SIVA1 fluorescence intensity/cell. Data were analyzed using unpaired 2-tailed t-test. Data are displayed as mean +- SD.
